Andrea Martin, of Clinton, says she is paying for the $2,500 operation out of her own pocket. She says the alternative is euthanization.
The chicken, named Cecily, was born with a damaged tendon in the leg that makes it useless.
The surgery will begin when Emi Knafo, a specialist in avian orthopedics, will amputate Cecily's right leg.
After a 10- to 14-day recovery period, the prosthetic, made on a 3-D printer, will be fitted.
Similar surgery has been performed on a rooster and duck, though not at Tufts.